Journal: Cell reports

Article Title: MITF, TFEB, and TFE3 drive distinct adaptive gene expression programs and immune infiltration in melanoma

doi: 10.1016/j.celrep.2025.116499

Figure Lengend Snippet: (A) Left, western blot of IGR37 melanoma cell line transfected with control or MITF-specific small interfering RNA (siRNA). Right, RT-qPCR for PTEN mRNA from corresponding IGR37 cells. (B and C) Heatmaps showing relative mRNA expression in CCLE melanoma or Tsoi et al. melanoma cell lines. (B) Right, Pearson correlation of gene expression. (D) TCGA melanomas ranked by MITF expression ( MITF ; black line). Gray bars indicate expression of RRAGD or FNIP2 in each melanoma, with the moving average of each per 20 melanoma window indicated by colored lines. (E) MITF ChIP-seq showing binding to the RRAGD or FNIP2 genes. (F) Western blot of melanoma cell lines. (G and H) Box and whisker plots showing relative expression of MITF , RRAGD , and FNIP2 based on triplicate RNA-seq of IGR37 cells expressing doxycycline-inducible β-catenin (iCTNNB1) (G) or in MeWo cells after short hairpin RNA (shRNA)-mediated depletion of MITF (H). See also .

Article Snippet: MeWo Human melanoma cell line (male) , Obtained from the ATCC , Cornil et al. .

Techniques: Western Blot, Transfection, Control, Small Interfering RNA, Quantitative RT-PCR, Expressing, Gene Expression, ChIP-sequencing, Binding Assay, Whisker Assay, RNA Sequencing, shRNA